New

Protein Science Principal Research Associate

Vertex Pharmaceuticals Incorporated
paid time off, 401(k)
United States, Washington, Seattle
Jan 14, 2026
Job Description Protein Science Principal Research Associate (Protein Engineering, Protein Production) General/Position Summary The Protein Sciences Principal Research Associate provides laboratory support in advancement of Protein Engineering pipeline with conducting cloning workflows and plasmid preparation. They work closely with protein engineers to provide support with yeast transformation, yeast cultures and characterization by flow cytometry. They provide support for mammalian expressions and high-throughput purification and biophysical characterization of proteins as needed. Key Duties & Responsibilities Execute and troubleshoot molecular cloning workflows spanning plasmid assembly to plasmid preparation at mini- and midi- scales Perform small-scale yeast transformations and culturing Support characterization of yeast displayed proteins by flow cytometry Support large-scale yeast transformations with DNA libraries Support lab operations and equipment purchasing if needed Perform high-throughput purification of proteins and protein-analytics using UV-Vis Spectroscopy, analytical SEC (UPLC-HPLC through Empower software), endotoxin tests, SDS-PAGE, and western blotting Manage protein inventory in the protein database and Protein Science freezers Provide protein dilutions and protein aliquots to immunology group and pharmacology group as needed Deliver verbal and presentation updates on cloning, expression, purification and analytics to the Protein Science Team. Ensure that a complete and timely record of experiments, constructs, plasmid preps is maintained in both the protein database and electronic lab notebooks Required Education Level Master's Degree (or equivalent degree) in cellular biology, biochemistry, biophysics, or bio/chemical engineering and 3+ years of relevant industry experience, or Bachelor's Degree (or equivalent degree) in cellular biology, biochemistry, biophysics, or bio/chemical engineering and 6 + years of relevant industry experience Required Knowledge/Skills Experience using electronic lab notebooks, cloning software such as snap gene Experience in working in a highly collaborative laboratory which is timeline-driven and result orientated team is a must Experience in mammalian cell culture wok to transiently express and purify recombinant proteins is a must Expertise in molecular biology techniques such as PCR, DNA cloning is a must Pay Range: $88,000 - $132,000 Disclosure Statement: The range provided is based on what we believe is a reasonable estimate for the base salary pay range for this job at the time of posting. This role is eligible for an annual bonus and annual equity awards. Some roles may also be eligible for overtime pay, in accordance with federal and state requirements. Actual base salary pay will be based on a number of factors, including skills, competencies, experience, and other job-related factors permitted by law. At Vertex, our Total Rewards offerings also include inclusive market-leading benefits to meet our employees wherever they are in their career, financial, family and wellbeing journey while providing flexibility and resources to support their growth and aspirations. From medical, dental and vision benefits to generous paid time off (including a week-long company shutdown in the Summer and the Winter), educational assistance programs including student loan repayment, a generous commuting subsidy, matching charitable donations, 401(k) and so much more. Flex Designation: On-Site Designated Flex Eligibility Status: In this On-Site designated role, you will work five days per week on-site with ad hoc flexibility. Note: The Flex status for this position is subject to Vertex's Policy on Flex @ Vertex Program and may be changed at any time. #LI-Onsite Company Information Vertex is a global biotechnology company that invests in scientific innovation. Vertex is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry, ethnicity, disability, veteran status, genetic information, sexual orientation, marital status, or any characteristic protected under applicable law. Vertex is an E-Verify Employer in the United States. Vertex will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law. Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the recruiter or hiring manager, or contact Talent Acquisition at ApplicationAssistance@vrtx.com