Research Associate - Ophthalmology - 139234

The Viterbi Family Department of Ophthalmology and Shiley Eye Institute at the University of California San Diego (UCSD) is an internationally recognized center conducting basic, translational and clinical science research on eye diseases.

The Borooah Lab at UC San Diego is seeking a highly motivated Research Associate to join our collaborative research team. Our lab conducts translational research aimed at developing novel treatments for retinal diseases. This position offers the opportunity to gain hands-on experience in stem cell disease modeling, gene therapy, CRISPR-based gene editing, antisense oligonucleotide (ASO) therapy, and regenerative medicine in an academic research setting.

Under supervision, the Research Associate will support research studies focused on understanding the molecular mechanisms underlying eye diseases and supporting the development of new therapeutic approaches. The role is primarily hands-on and involves learning and assisting in core molecular and cellular biology techniques, including DNA and RNA extraction, PCR and qPCR, Western blotting, and immunocytochemistry. A key component of this position includes working with cell cultures, such as assisting with the maintenance of induced pluripotent stem cell (iPSC) lines and supporting the differentiation and use of 3D retinal organoids as disease models.

Additional responsibilities include following established laboratory protocols with attention to detail, assisting in the optimization of experimental workflows, and assisting with collecting, organizing, and documenting data for research reports, publications, and grant applications. The Research Associate will also help maintain research records and databases, support general laboratory organization and upkeep, and assist with the training of students or new lab members as needed. Other duties as assigned.

*Theoretical knowledge of a life science as is typically acquired by a Bachelor's Degree in a field related to the research being conducted such as biology, genetics, molecular biology or biochemistry or an equivalent combination of education and experience.

*Knowledge and experience with basic and general techniques in molecular biology.

*Ability to work with minimum supervision and to work closely and collaboratively with others including the ability to follow written and oral instructions.

*Ability to prepare solutions at proper concentrations and perform accurate calculations.

*Ability to communicate clearly and interact with colleagues and collaborators.

*Ability to read and interpret protocols and policies.

*Experience with personal computers. Ability to use database and spreadsheet.

• Experience in general laboratory techniques and molecular techniques; biochemistry, molecular biology, basic genetics.

• Experience with measuring, weighing, and mixing chemicals.

• Experience in sterile technique to culture cells.

• Must be willing to work in patient and animal-related research environment.

• Job offer is contingent upon satisfactory clearance based on Background Check results.