Principal Scientist

Overview

The Bioanalytical Sciences group at Alnylam is seeking a highly motivated Senior Scientist to develop and implement fit-for-purpose methods across diverse ligand binding and molecular platforms to support bioanalysis of proteins, peptides, antibodies, RNA, and oligonucleotides to inform target engagement of new chemistries and delivery modalities across preclinical species and biological matrices.

You will work in a matrixed environment, partnering closely with Investigative Sciences, Research, and Chemistry leads to establish and advance internal bioanalytical assays for emerging tissues. By analyzing and interpreting results, you will help drive critical insights into mechanism of action, safety, and efficacy, directly shaping decision-making in RNAi therapeutic development.

You have a strong foundation in biochemistry and molecular biology and a proven ability to thrive in fast-paced, high-intensity, time-sensitive environments. You are flexible and adaptable, and thrive on meeting quick turnaround times. You are a creative problem solver, an effective communicator, and thrive in collaborative, team-oriented settings.

This is a unique opportunity to join a vibrant, cross-functional team, working alongside highly motivated scientists from a wide range of disciplines. If you're driven, adaptable, and eager to contribute to impactful science, we'd love to hear from you.

This position is onsite and will be primarily located in Cambridge, MA.

Key Responsibilities

• Apply expertise and innovative problem-solving to design, optimize, and troubleshoot ligand binding (ELISA, MSD, Quanterix SIMOA, ARGO NULISA) and molecular assays (ddPCR, qPCR), adapting methods creatively for complex preclinical models and biological matrices
• Repurpose existing methods to support rapid sample analysis, leveraging comparisons to historical data to inform next steps for platform optimization and delivery advancement
• Provide Subject Matter Expertise in ligand binding and molecular assays, with deep understanding in technologies and applications to assess strengths and limitations
• Synthesize internal and published data to inform on and execution of assays
• Create training materials and SOPs; mentor analysts and drive successful internal/external method transfers with defined acceptance criteria
• Collaborate with cross-functional teams within Research and Development to align on projects and timelines, and provide scientific oversight on bioanalytical data that support context of use
• Identify, assess, and implement cutting-edge technologies to strengthen assay performance, expand technical capabilities, and improve operational efficiency.
• Maintain laboratory notebooks, perform data analysis and QC, and communicate results to cross-functional teams and external science forums.

Qualifications

• Ph.D. in Biochemistry, Molecular/Cell Biology, or related field with 5+ years of relevant industry experience; or exceptional M.S. with 8+ years of experience.
• In-depth knowledge and hands-on expertise with ligand binding platforms (ELISA, MSD, Quanterix) and molecular platforms (Applied Biosystems/Bio-Rad).
• Familiarity with discovery-based platforms (OLINK, OpenArray, QuantiGene) is a plus.
• Proficient in Microsoft Office (Excel, Word, PowerPoint) and skilled with specialized analytical platforms (GraphPad Prism, SoftMax Pro, Discovery Workbench, QuantStudio) to support data analysis, visualization, and reporting.
• Strong understanding of disease biology and the drug development process, enabling the design and implementation of fit-for-purpose assays.
• Self-motivated, detail-oriented, and highly organized, with the ability to manage multiple projects in a fast-paced, collaborative environment.
• Demonstrated track record of scientific communication, including conference presentations, peer-reviewed publications, and/or regulatory or industry white papers.
• Excellent interpersonal, verbal, and written communication skills to support effective cross-functional collaboration.

U.S. Pay Range

$148,200.00 - $208,800.00

The pay range reflects the full-time base salary range we expect to pay for this role at the time of posting. Base pay will be determined based on a number of factors including, but not limited to, relevant experience, skills, and education. This role is eligible for an annual short-term incentive award (e.g., bonus or sales incentive) and an annual long-term incentive award (e.g., equity).

Alnylam's robust Total Rewards package is designed to support your overall health and well-being. We offer comprehensive benefits including medical, dental, and vision coverage, life and disability insurance, a lifestyle reimbursement program, flexible spending and health savings accounts and a 401(k)with a generous company match. Eligible employees enjoy paid time off, wellness days, holidays, and two company-wide recharge breaks. We also offer generous family resources and leave. Our commitment to your well-being reflects our belief that caring for our people fuels the impact we create together.

Learn more about these and additional benefits offered by Alnylam by visiting the Benefits section of the Careers website: https://www.alnylam.com/careers

AboutAlnylam

We are the leader in RNAi therapeutics- a revolutionaryapproach with the potential to transform the lives of people with rare and common diseases. Built on Nobel Prize-winning science, Alnylam has delivered the breakthroughs that made RNAi therapeutics possibleand are just at the beginning of what's possible. Our deep pipeline, late-stage programs, and bold vision reflect our core values: fierce innovation, passion for excellence, purposeful urgency, open culture and commitment to people. We're proud to be a globally recognized top employer, wherean authentic, inclusive culture and breakthrough thinkingfuel one another.

At Alnylam, we commit to an inclusive recruitment process and equal employment opportunity. Qualified applicants will receive consideration for employment without regard to their sex, gender or gender identity, sexual orientation, race, color, ethnicity, national origin, ancestry, citizenship, religion, creed, physical or mental disability, pregnancy status or related conditions, genetic information, veteran or military status, marital or familial status, political affiliation, age, or any other factor protected by federal, state, or local law. Alnylam is an E-Verify Employer.